Hotspot

psaA-ycf3

LSC · 41184-42003 · 820 bp

Back to Atriplex

Pi 0.0117

MarkerSeek score 57.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 19
pi: 0.0117
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAAAAATGACCGGGTCTGG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.1	57.9	943-951 bp	1.0	80.6
psaA-ycf3_p2	TTTTACTTCTGGTTCCGGCG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.5	57.9	877-885 bp	1.0	77.6
psaA-ycf3_p3	GTCTGGCCCATTCCTCAAAC	ATTGGTTGAAGATCACGGGG	55.0	50.0	58.8	57.9	929-937 bp	1.0	74.8
psaA-ycf3_p4	CGAAGTTTTTACGGGATCCCT	ATTGGTTGAAGATCACGGGG	47.6	50.0	58.0	57.9	910-918 bp	1.0	73.3
psaA-ycf3_p5	GGCCCATTCCTCAAACGAA	ATTGGTTGAAGATCACGGGG	52.6	50.0	58.0	57.9	925-933 bp	1.0	73.0