Hotspot

psbC-trnS-UGA

LSC · 34539-34788 · 250 bp

Back to Atriplex

Pi 0.0268

MarkerSeek score 56.70

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 250
variable_sites: 13
pi: 0.0268
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCGATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.3	58.1	357 bp	1.0	80.4
psbC-trnS-UGA_p2	CTGTTCTTTCGATGACCCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.3	58.1	356 bp	1.0	80.4
psbC-trnS-UGA_p3	CCTGTTCTTTCGATGACCCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	58.3	58.2	303 bp	1.0	79.6
psbC-trnS-UGA_p4	CTGTTCTTTCGATGACCCCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	58.3	58.2	302 bp	1.0	79.6
psbC-trnS-UGA_p5	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.4	58.1	400 bp	1.0	79.1