Hotspot

trnT-GGU-psbD

LSC · 30849-32107 · 1259 bp

Back to Atriplex

Pi 0.00739

MarkerSeek score 61.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1259
variable_sites: 19
pi: 0.00739
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	AGACCAACCGACAAAAACGA	55.0	45.0	58.1	58.0	1403-1419 bp	1.0	81.7
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	AGACCAACCGACAAAAACGA	50.0	45.0	58.1	58.0	1406-1422 bp	1.0	81.5
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	ATAGTCCAGACCAACCGACA	55.0	50.0	58.1	58.1	1410-1426 bp	1.0	81.4
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	ATAGTCCAGACCAACCGACA	50.0	50.0	58.1	58.1	1413-1429 bp	1.0	81.2
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CAGACCAACCGACAAAAACG	55.0	50.0	58.1	57.9	1404-1420 bp	1.0	81.1