Hotspot

psbM-trnD-GUC

LSC · 28852-29605 · 754 bp

Back to Atriplex

Pi 0.0138

MarkerSeek score 62.20

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 754
variable_sites: 21
pi: 0.0138
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TCATTCTGGCTAACCGTTTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.3	58.6	904-918 bp	1.0	57.1
psbM-trnD-GUC_p2	TCATTCTGGCTAACCGTTTTT	TCAATTGGTTAGAGCACCGC	38.1	50.0	56.1	58.6	904-918 bp	1.0	55.6
psbM-trnD-GUC_p3	AATCATTCTGGCTAACCGTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.0	58.6	906-920 bp	1.0	55.2
psbM-trnD-GUC_p4	ATCATTCTGGCTAACCGTTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.0	58.6	905-919 bp	1.0	55.2
psbM-trnD-GUC_p5	TCTGGCTAACCGTTTTTACA	TCAATTGGTTAGAGCACCGC	40.0	50.0	54.9	58.6	900-914 bp	1.0	54.4