Hotspot

petN-psbM

LSC · 27890-28746 · 857 bp

Back to Atriplex

Pi 0.0216

MarkerSeek score 65.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 857
variable_sites: 40
pi: 0.0216
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATTGTAAGTCTCGCTTGGGC	AAAACGGTTAGCCAGAATGA	50.0	40.0	58.3	55.3	965-966 bp	1.0	59.4
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	AAAACGGTTAGCCAGAATGA	50.0	40.0	57.6	55.3	903-904 bp	1.0	58.9
petN-psbM_p3	ATTGTAAGTCTCGCTTGGGC	AAAAACGGTTAGCCAGAATGA	50.0	38.1	58.3	56.1	966-967 bp	1.0	57.8
petN-psbM_p4	ATTGTAAGTCTCGCTTGGGC	AAACGGTTAGCCAGAATGAT	50.0	40.0	58.3	55.0	964-965 bp	1.0	57.5
petN-psbM_p5	ATTGTAAGTCTCGCTTGGGC	AACGGTTAGCCAGAATGATT	50.0	40.0	58.3	55.0	963-964 bp	1.0	57.5