Hotspot

trnC-GCA-petN

LSC · 27298-27799 · 502 bp

Back to Atriplex

Pi 0.0143

MarkerSeek score 59.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 502
variable_sites: 14
pi: 0.0143
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TGTTCCCCAGTTCAAATCCG	GCCCAAGCGAGACTTACAAT	50.0	50.0	58.1	58.3	550-559 bp	1.0	80.3
trnC-GCA-petN_p2	TGTTCCCCAGTTCAAATCCG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	612-621 bp	1.0	79.3
trnC-GCA-petN_p3	ACTGCAAATCCTTGTTCCCC	GCCCAAGCGAGACTTACAAT	50.0	50.0	58.4	58.3	562-571 bp	1.0	78.1
trnC-GCA-petN_p4	GCAAATCCTTGTTCCCCAGT	GCCCAAGCGAGACTTACAAT	50.0	50.0	58.4	58.3	559-568 bp	1.0	78.1
trnC-GCA-petN_p5	TGCAAATCCTTGTTCCCCAG	GCCCAAGCGAGACTTACAAT	50.0	50.0	58.4	58.3	560-569 bp	1.0	78.1