Hotspot

rpoB-trnC-GCA

LSC · 26101-27226 · 1126 bp

Back to Atriplex

Pi 0.0124

MarkerSeek score 58.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1126
variable_sites: 28
pi: 0.0124
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCATTTCCATCCCGTAGCAT	CGGATTTGAACTGGGGAACA	50.0	50.0	57.7	58.1	1207-1214 bp	1.0	79.1
rpoB-trnC-GCA_p2	CATTCCCCCATTTCCATCCC	CGGATTTGAACTGGGGAACA	55.0	50.0	58.3	58.1	1214-1221 bp	1.0	79.1
rpoB-trnC-GCA_p3	CCATTTCCATCCCGTAGCAT	ACAAGGATTTGCAGTCCTCC	50.0	50.0	57.7	57.8	1190-1197 bp	1.0	78.3
rpoB-trnC-GCA_p4	CATTCCCCCATTTCCATCCC	ACAAGGATTTGCAGTCCTCC	55.0	50.0	58.3	57.8	1197-1204 bp	1.0	78.2
rpoB-trnC-GCA_p5	ACATTCCCCCATTTCCATCC	CGGATTTGAACTGGGGAACA	50.0	50.0	57.5	58.1	1215-1222 bp	1.0	77.5