Hotspot

rpl2

IRb · 18887-20376 · 1490 bp

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Pi NA

MarkerSeek score 30.20

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRb
length_bp: 1490
variable_sites: 0
pi: NA
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 30.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	CGCTTCAACCGGGTTATTCT	GTGAACGTGTCACGGTTAAT	50.0	45.0	58.3	56.4	1630-1635 bp	1.0	69.1
rpl2_p2	ATCATTACGCTTCAACCGGG	GTGAACGTGTCACGGTTAAT	50.0	45.0	58.3	56.4	1637-1642 bp	1.0	68.6
rpl2_p3	CGCTTCAACCGGGTTATTCT	GTGAACGTGTCACGGTTAATT	50.0	42.9	58.3	57.1	1630-1635 bp	1.0	67.0
rpl2_p4	ACGCTTCAACCGGGTTATTC	GTGAACGTGTCACGGTTAAT	50.0	45.0	58.6	56.4	1631-1636 bp	1.0	66.8
rpl2_p5	ATCATTACGCTTCAACCGGG	GTGAACGTGTCACGGTTAATT	50.0	42.9	58.3	57.1	1637-1642 bp	1.0	66.4