Hotspot

rpl14-rpl16

LSC · 106490-106607 · 118 bp

Back to Atraphaxis

Pi 0.0161

MarkerSeek score 52.50

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 118
variable_sites: 3
pi: 0.0161
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ACACATCAATTCTCGAGCCC	GTCAAACCGGGTCGAATACT	50.0	50.0	58.0	57.6	299-341 bp	1.0	78.3
rpl14-rpl16_p2	ACACATCAATTCTCGAGCCC	AATAGCGGCATCCAAAATGC	50.0	45.0	58.0	57.5	231-273 bp	1.0	77.6
rpl14-rpl16_p3	GACGGTTGCTAGCTCCTATG	GTCAAACCGGGTCGAATACT	55.0	50.0	57.9	57.6	327-369 bp	1.0	77.3
rpl14-rpl16_p4	ACGGTTGCTAGCTCCTATGA	GTCAAACCGGGTCGAATACT	50.0	50.0	58.2	57.6	326-368 bp	1.0	76.6
rpl14-rpl16_p5	GACGGTTGCTAGCTCCTATG	AATAGCGGCATCCAAAATGC	55.0	45.0	57.9	57.5	259-301 bp	1.0	76.5