Hotspot

trnF-GAA-ndhJ

LSC · 72850-73538 · 689 bp

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Pi 0.00849

MarkerSeek score 55.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 689
variable_sites: 16
pi: 0.00849
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TCCACGCCTGAAACGTATTT	50.0	45.0	58.1	57.8	820-826 bp	1.0	80.4
trnF-GAA-ndhJ_p2	AGTAGATGGTGCGTAGGGAA	TCCACGCCTGAAACGTATTT	50.0	45.0	57.8	57.8	881-887 bp	1.0	79.5
trnF-GAA-ndhJ_p3	AATGGTCGGGATAGCTCAGT	TCCACGCCTGAAACGTATTT	50.0	45.0	58.2	57.8	863-869 bp	1.0	79.1
trnF-GAA-ndhJ_p4	ATGGTCGGGATAGCTCAGTT	TCCACGCCTGAAACGTATTT	50.0	45.0	58.2	57.8	862-868 bp	1.0	79.1
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	CATCCACGCCTGAAACGTAT	50.0	50.0	58.1	58.3	822-828 bp	1.0	79.0