Hotspot

psbZ-trnG-UCC

LSC · 60019-60325 · 307 bp

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Pi 0.00762

MarkerSeek score 55.50

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 307
variable_sites: 6
pi: 0.00762
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TGTCGTATTTGCGTCTCCTG	CATCTTGAACCTTGAGCGGA	50.0	50.0	58.0	57.9	502-510 bp	1.0	81.4
psbZ-trnG-UCC_p2	TGTCGTATTTGCGTCTCCTG	TCATCTTGAACCTTGAGCGG	50.0	50.0	58.0	57.9	503-511 bp	1.0	81.4
psbZ-trnG-UCC_p3	TGTCGTATTTGCGTCTCCTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.0	57.8	464-472 bp	1.0	81.0
psbZ-trnG-UCC_p4	GTCGTATTTGCGTCTCCTGA	CATCTTGAACCTTGAGCGGA	50.0	50.0	57.7	57.9	501-509 bp	1.0	79.2
psbZ-trnG-UCC_p5	GTCGTATTTGCGTCTCCTGA	TCATCTTGAACCTTGAGCGG	50.0	50.0	57.7	57.9	502-510 bp	1.0	79.2