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petN-psbM

LSC · 50945-52123 · 1179 bp

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Pi 0.00566

MarkerSeek score 62.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1179
variable_sites: 15
pi: 0.00566
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	CCCTTTCACTCGTCGTATGG	TGCTACTGCACTCTTCATTCT	55.0	42.9	58.1	57.0	1268-1296 bp	1.0	66.4
petN-psbM_p2	CCTTTCACTCGTCGTATGGG	TGCTACTGCACTCTTCATTCT	55.0	42.9	58.1	57.0	1267-1295 bp	1.0	66.4
petN-psbM_p3	CTTTCACTCGTCGTATGGGG	TGCTACTGCACTCTTCATTCT	55.0	42.9	58.1	57.0	1266-1294 bp	1.0	66.4
petN-psbM_p4	GTATGGGGGAGAAGTGGACT	TGCTACTGCACTCTTCATTCT	55.0	42.9	58.1	57.0	1254-1282 bp	1.0	66.1
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTCTTCATTCT	50.0	42.9	58.2	57.0	1313-1341 bp	1.0	65.7