Pi
0.000654
MarkerSeek score
55.20
Primer available
yes
Label
ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf1_p1 | ACTAGATTGTGTAATGCTGAGACT | TGTGTTGAGTAAAAACCTCT | 37.5 | 35.0 | 57.5 | 52.2 | 357 bp | 1.0 | 40.9 |
| ycf1_p2 | ACTAGATTGTGTAATGCTGAGACT | TGTGTTGAGTAAAAACCTCTT | 37.5 | 33.3 | 57.5 | 53.2 | 357 bp | 1.0 | 40.9 |
| ycf1_p3 | ACTAGATTGTGTAATGCTGAGACT | TTGTGTTGAGTAAAAACCTCT | 37.5 | 33.3 | 57.5 | 53.2 | 358 bp | 1.0 | 40.9 |
| ycf1_p4 | ACTAGATTGTGTAATGCTGAGACT | TTGTGTTGAGTAAAAACCTCTT | 37.5 | 31.8 | 57.5 | 54.1 | 358 bp | 1.0 | 40.9 |
| ycf1_p5 | ACTAGATTGTGTAATGCTGAGACT | TTTGTGTTGAGTAAAAACCTCT | 37.5 | 31.8 | 57.5 | 54.1 | 359 bp | 1.0 | 40.9 |
| ycf1_p1 | GGGTGGGCGTATTTCTTCTT | AGAAAAGAAAAAGGGAGGTTTGTG | 50.0 | 37.5 | 57.9 | 58.0 | 674-697 bp | 1.0 | 49.6 |
| ycf1_p2 | GGGTGGGCGTATTTCTTCTT | AAGAAAAAGGGAGGTTTGTG | 50.0 | 40.0 | 57.9 | 53.9 | 670-693 bp | 1.0 | 48.9 |
| ycf1_p3 | GGGTGGGCGTATTTCTTCTT | AAAGAAAAAGGGAGGTTTGTG | 50.0 | 38.1 | 57.9 | 54.8 | 671-694 bp | 1.0 | 47.8 |
| ycf1_p4 | GGGTGGGCGTATTTCTTCTT | AGAAAAGAAAAAGGGAGGTTTGT | 50.0 | 34.8 | 57.9 | 56.7 | 674-697 bp | 1.0 | 47.6 |
| ycf1_p5 | GGGTGGGCGTATTTCTTCTT | GAAAAGAAAAAGGGAGGTTTGTG | 50.0 | 39.1 | 57.9 | 56.7 | 673-696 bp | 1.0 | 47.4 |