Hotspot

rrn4.5S

IRb · 107205-107469 · 265 bp

Back to Atractylodes

Pi 0.00226

MarkerSeek score 35.20

Primer available yes

Label rrn4.5S

Feature Metadata

label_name: rrn4.5S
feature_type: rRNA
region: IRb
length_bp: 265
variable_sites: 1
pi: 0.00226
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5S_p1	TCTCCTATTCCGACTTCCCC	ACGCCTAGGACACCAGAATA	55.0	50.0	57.9	57.8	472 bp	1.0	78.3
rrn4.5S_p2	TCTCCTATTCCGACTTCCCC	TACGCCTAGGACACCAGAAT	55.0	50.0	57.9	57.8	473 bp	1.0	78.3
rrn4.5S_p3	GGATGGAGCGACAGAAGTTT	ACGCCTAGGACACCAGAATA	50.0	50.0	57.9	57.8	401 bp	1.0	78.2
rrn4.5S_p4	GGATGGAGCGACAGAAGTTT	TACGCCTAGGACACCAGAAT	50.0	50.0	57.9	57.8	402 bp	1.0	78.2
rrn4.5S_p5	TCTCCTATTCCGACTTCCCC	TTCCTCTACGCCTAGGACAC	55.0	55.0	57.9	58.2	479 bp	1.0	77.6