Hotspot

rpoB

LSC · 12996-16178 · 3183 bp

Back to Atractylodes

Pi 0.000754

MarkerSeek score 48.40

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3183
variable_sites: 6
pi: 0.000754
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	CTTTGGCCTTGAATCAGGGT	ACTGAGAAAGAGGGACTGCT	50.0	50.0	58.1	58.0	222 bp	1.0	80.0
rpoB_p2	CTTTGGCCTTGAATCAGGGT	GCCTTCCAATCCAGTTCCAA	50.0	50.0	58.1	58.1	257 bp	1.0	79.4
rpoB_p3	CTTTGGCCTTGAATCAGGGT	TTGGCCTTCCAATCCAGTTC	50.0	50.0	58.1	58.1	260 bp	1.0	79.4
rpoB_p4	CTTTGGCCTTGAATCAGGGT	CAATGCACTTCTCGGACTGA	50.0	50.0	58.1	57.9	237 bp	1.0	79.4
rpoB_p5	CTTTGGCCTTGAATCAGGGT	GAGAAAGAGGGACTGCTTGG	50.0	55.0	58.1	57.9	219 bp	1.0	79.3