Hotspot

clpP

LSC · 70062-72075 · 2014 bp

Back to Atractylodes

Pi 0.000199

MarkerSeek score 50.40

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2014
variable_sites: 1
pi: 0.000199
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CGAATTGACGCGAAATCCAG	GCCTTTGTTACGTTTCCACA	50.0	45.0	57.9	56.9	2101-2103 bp	1.0	70.4
clpP_p2	CTCGGCAGGGGTAATTTCAA	GCCTTTGTTACGTTTCCACA	50.0	45.0	57.9	56.9	2125-2127 bp	1.0	70.0
clpP_p3	AACTCGGCAGGGGTAATTTC	GCCTTTGTTACGTTTCCACA	50.0	45.0	57.9	56.9	2127-2129 bp	1.0	70.0
clpP_p4	CACGAATTGACGCGAAATCC	GCCTTTGTTACGTTTCCACA	50.0	45.0	58.2	56.9	2103-2105 bp	1.0	69.4
clpP_p5	CGAATTGACGCGAAATCCAG	AGCCTTTGTTACGTTTCCACA	50.0	42.9	57.9	58.4	2102-2104 bp	1.0	68.7