Hotspot

ndhC-trnM-CAT

LSC · 50329-52337 · 2009 bp

Back to Atractylodes

Pi 0.000796

MarkerSeek score 51.10

Primer available yes

Label ndhC-trnM-CAT

Feature Metadata

label_name: ndhC-trnM-CAT
feature_type: igs
region: LSC
length_bp: 2009
variable_sites: 4
pi: 0.000796
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM-CAT_p1	GTCCTTTGCTAATCGGAGCT	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.7	58.4	2164-2171 bp	1.0	74.5
ndhC-trnM-CAT_p2	GTCCTTTGCTAATCGGAGCT	ATACCGCGGTCAATGGTATC	50.0	50.0	57.7	57.5	2223-2230 bp	1.0	74.2
ndhC-trnM-CAT_p3	GTCCTTTGCTAATCGGAGCT	CCGCGGTCAATGGTATCTAA	50.0	50.0	57.7	57.5	2220-2227 bp	1.0	73.6
ndhC-trnM-CAT_p4	GTCCTTTGCTAATCGGAGCT	TTAGATACCGCGGTCAATGG	50.0	50.0	57.7	57.5	2227-2234 bp	1.0	73.6
ndhC-trnM-CAT_p5	CGGTCCTTTGCTAATCGGAG	CCCGCCGTATGAAAGCAATA	55.0	50.0	58.4	58.4	2166-2173 bp	1.0	73.6