Hotspot

trnR-TCT-trnT-GGT

LSC · 30025-31173 · 1149 bp

Back to Atractylodes

Pi 0.00174

MarkerSeek score 52.50

Primer available yes

Label trnR-TCT-trnT-GGT

Feature Metadata

label_name: trnR-TCT-trnT-GGT
feature_type: igs
region: LSC
length_bp: 1149
variable_sites: 4
pi: 0.00174
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-TCT-trnT-GGT_p1	AGAAGACCTCTGTCCTATCCA	ATTTGAACCGAGGGCTTACG	47.6	50.0	56.9	58.3	1244-1246 bp	1.0	61.7
trnR-TCT-trnT-GGT_p2	AGGTTTAGAAGACCTCTGTCCT	ATTTGAACCGAGGGCTTACG	45.5	50.0	57.9	58.3	1250-1252 bp	1.0	61.0
trnR-TCT-trnT-GGT_p3	AGAAGACCTCTGTCCTATCCA	GGATTTGAACCGAGGGCTTA	47.6	50.0	56.9	57.6	1246-1248 bp	1.0	60.5
trnR-TCT-trnT-GGT_p4	AGGTTTAGAAGACCTCTGTCCT	GGATTTGAACCGAGGGCTTA	45.5	50.0	57.9	57.6	1252-1254 bp	1.0	59.9
trnR-TCT-trnT-GGT_p5	AGAAGACCTCTGTCCTATCCAT	ATTTGAACCGAGGGCTTACG	45.5	50.0	57.4	58.3	1244-1246 bp	1.0	57.7