Hotspot

atpH-atpF

LSC · 26595-26968 · 374 bp

Back to Atractylodes

Pi 0.00374

MarkerSeek score 36.90

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 374
variable_sites: 3
pi: 0.00374
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	GCCTGGTTGTAGCATTAGCA	TGGCCCAAAGAAACGAAAGA	50.0	45.0	58.2	57.9	459 bp	1.0	78.0
atpH-atpF_p2	GCCTGGTTGTAGCATTAGCA	ATTAAACCCGAAACTCCCGG	50.0	50.0	58.2	57.9	490 bp	1.0	77.5
atpH-atpF_p3	GCCTGGTTGTAGCATTAGCA	GTGGCCCAAAGAAACGAAAG	50.0	50.0	58.2	57.9	460 bp	1.0	77.4
atpH-atpF_p4	TGGCCTGGTTGTAGCATTAG	TGGCCCAAAGAAACGAAAGA	50.0	45.0	57.6	57.9	461 bp	1.0	76.7
atpH-atpF_p5	GCCTGGTTGTAGCATTAGCA	AGTGGCCCAAAGAAACGAAA	50.0	45.0	58.2	58.2	461 bp	1.0	76.7