Hotspot

rpoC1-rpoC2

LSC · 19014-19133 · 120 bp

Back to Atractylodes

Pi 0.00333

MarkerSeek score 49.10

Primer available yes

Label rpoC1-rpoC2

Feature Metadata

label_name: rpoC1-rpoC2
feature_type: igs
region: LSC
length_bp: 120
variable_sites: 1
pi: 0.00333
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoC2_p1	CTATGCTACCAGCGGGAATG	TAAAAGACATGAGTGGCCCG	55.0	50.0	58.2	57.9	181-199 bp	1.0	82.0
rpoC1-rpoC2_p2	ATTCTATGCTACCAGCGGGA	TAAAAGACATGAGTGGCCCG	50.0	50.0	58.3	57.9	184-202 bp	1.0	81.3
rpoC1-rpoC2_p3	TCTATGCTACCAGCGGGAAT	TAAAAGACATGAGTGGCCCG	50.0	50.0	58.3	57.9	182-200 bp	1.0	81.3
rpoC1-rpoC2_p4	CTATGCTACCAGCGGGAATG	GTTGTGCCATCAGTACCTCC	55.0	55.0	58.2	58.3	162-180 bp	1.0	80.8
rpoC1-rpoC2_p5	TCTATGCTACCAGCGGGAAT	GTTGTGCCATCAGTACCTCC	50.0	55.0	58.3	58.3	163-181 bp	1.0	80.1