Hotspot

trnE-TTC-rpoB

LSC · 12096-12995 · 900 bp

Back to Atractylodes

Pi 0.00089

MarkerSeek score 61.00

Primer available yes

Label trnE-TTC-rpoB

Feature Metadata

label_name: trnE-TTC-rpoB
feature_type: igs
region: LSC
length_bp: 900
variable_sites: 2
pi: 0.00089
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-TTC-rpoB_p1	AAGTCGTAACCTCTGGACCA	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.0	56.8	997-1007 bp	1.0	63.4
trnE-TTC-rpoB_p2	GGGAATTGAACCCTCGTTGT	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.1	56.8	1032-1042 bp	1.0	62.6
trnE-TTC-rpoB_p3	AAGTCGTAACCTCTGGACCA	TCAAACCCTGATCAATGAACCT	50.0	40.9	58.0	57.6	987-997 bp	1.0	62.0
trnE-TTC-rpoB_p4	AAGTCGTAACCTCTGGACCA	TCTTCTGTCAAACCCTGATCA	50.0	42.9	58.0	56.6	994-1004 bp	1.0	61.7
trnE-TTC-rpoB_p5	GAACCCTCGTTGTCTCAGTG	AGTTCTTCTGTCAAACCCTGA	55.0	42.9	58.2	56.8	1025-1035 bp	1.0	61.6