Hotspot

rpl32-ndhF

SSC · 124803-125855 · 1053 bp

Back to Atractylis

Pi 0.00382

MarkerSeek score 61.40

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1053
variable_sites: 4
pi: 0.00382
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CCGCCCAATATCCTTTCCTT	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	57.6	58.0	1233-1236 bp	1.0	77.4
rpl32-ndhF_p2	CCGCCCAATATCCTTTCCTT	TGCCGTCGGAAAAAGAAGAA	50.0	45.0	57.6	58.0	1231-1234 bp	1.0	77.3
rpl32-ndhF_p3	CCGCCCAATATCCTTTCCTT	TTGCCGTCGGAAAAAGAAGA	50.0	45.0	57.6	58.0	1232-1235 bp	1.0	77.3
rpl32-ndhF_p4	TGACAAAGCTTTTAACGCCG	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.6	58.0	1250-1253 bp	1.0	77.2
rpl32-ndhF_p5	TGACAAAGCTTTTAACGCCG	TGCCGTCGGAAAAAGAAGAA	45.0	45.0	57.6	58.0	1248-1251 bp	1.0	77.1