Hotspot

trnL-UAG-rpl32

SSC · 123784-124637 · 854 bp

Back to Atractylis

Pi 0.00468
MarkerSeek score 61.40
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
854
variable_sites
4
pi
0.00468
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC AAGGAAAGGATATTGGGCGG 50.0 50.0 58.0 57.6 986-1008 bp 1.0 77.3
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA AAGGAAAGGATATTGGGCGG 50.0 50.0 58.0 57.6 987-1009 bp 1.0 77.3
trnL-UAG-rpl32_p3 AGCGTGTCTACCGATTTCAC CGGCGTTAAAAGCTTTGTCA 50.0 45.0 58.0 57.6 969-991 bp 1.0 77.1
trnL-UAG-rpl32_p4 CAGCGTGTCTACCGATTTCA CGGCGTTAAAAGCTTTGTCA 50.0 45.0 58.0 57.6 970-992 bp 1.0 77.1
trnL-UAG-rpl32_p5 TCCTAAGAGCAGCGTGTCTA AAGGAAAGGATATTGGGCGG 50.0 50.0 57.9 57.6 996-1018 bp 1.0 76.4