Hotspot

rps19-rpl2

IRb · 84288-84346 · 59 bp

Back to Atractylis

Pi 0.0339

MarkerSeek score 68.20

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 59
variable_sites: 2
pi: 0.0339
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	GCCCGGGACCAGGTTATTAT	GGGCCCCAATTGGTAGAAAA	55.0	50.0	59.2	58.1	288-289 bp	1.0	70.3
rps19-rpl2_p2	GCCCGGGACCAGGTTATTAT	TTGATTCTTCGTCGCCGTAG	55.0	50.0	59.2	58.1	196-197 bp	1.0	70.2
rps19-rpl2_p3	GCCCGGGACCAGGTTATTAT	CACAACCCCTTGGGGTTATC	55.0	55.0	59.2	58.2	263-264 bp	1.0	69.5
rps19-rpl2_p4	CCCGGGACCAGGTTATTATT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	56.7	58.1	287-288 bp	1.0	69.4
rps19-rpl2_p5	CCCGGGACCAGGTTATTATT	TTGATTCTTCGTCGCCGTAG	50.0	50.0	56.7	58.1	195-196 bp	1.0	69.3