Hotspot

psbE-petL

LSC · 64671-65973 · 1303 bp

Back to Atractylis

Pi 0.00461

MarkerSeek score 55.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1303
variable_sites: 6
pi: 0.00461
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCCGTATCTTGCTCAGAC	50.0	50.0	57.9	57.7	1497-1510 bp	1.0	77.5
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AAGCCGTATCTTGCTCAGAC	50.0	50.0	57.8	57.7	1420-1433 bp	1.0	76.7
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AAGCCGTATCTTGCTCAGAC	45.0	50.0	58.2	57.7	1506-1519 bp	1.0	76.6
psbE-petL_p4	TGCTGACGAATAACCAACCC	AAGCCGTATCTTGCTCAGAC	50.0	50.0	58.2	57.7	1511-1524 bp	1.0	76.5
psbE-petL_p5	CGAATAACCAACCCGCAATG	AAGCCGTATCTTGCTCAGAC	50.0	50.0	57.8	57.7	1505-1518 bp	1.0	76.5