Hotspot

atpB-rbcL

LSC · 54118-54915 · 798 bp

Back to Atractylis

Pi 0.00501

MarkerSeek score 56.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 798
variable_sites: 4
pi: 0.00501
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTGGCGGAAAAGCTACATCT	CACCTGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	956-975 bp	1.0	72.0
atpB-rbcL_p2	CTGGCGGAAAAGCTACATCT	ACCTGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	955-974 bp	1.0	71.7
atpB-rbcL_p3	CTGGCGGAAAAGCTACATCT	TGCTTTGAATCCAACACTTGC	50.0	42.9	58.0	57.9	952-971 bp	1.0	71.3
atpB-rbcL_p4	GATACGCCCCAGGTTCTTTT	CACCTGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	913-932 bp	1.0	71.2
atpB-rbcL_p5	GATACGCCCCAGGTTCTTTT	ACCTGCTTTGAATCCAACACT	50.0	42.9	57.9	58.1	912-931 bp	1.0	70.9