Hotspot

psbM-trnD-GUC

LSC · 10793-11446 · 654 bp

Back to Atractylis

Pi 0.00306

MarkerSeek score 60.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 654
variable_sites: 2
pi: 0.00306
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GGAACTAAAATGAACAGTGCAGT	TGCTGCTAGATCCCTTCTGA	39.1	50.0	57.6	57.8	807-808 bp	1.0	51.5
psbM-trnD-GUC_p2	AAAATGAACAGTGCAGTAGC	TGCTGCTAGATCCCTTCTGA	40.0	50.0	54.5	57.8	801-802 bp	1.0	51.0
psbM-trnD-GUC_p3	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.6	58.3	770-771 bp	1.0	50.7
psbM-trnD-GUC_p4	AAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	40.0	50.0	54.5	58.3	764-765 bp	1.0	50.3
psbM-trnD-GUC_p5	ACTAAAATGAACAGTGCAGTAGC	TGCTGCTAGATCCCTTCTGA	39.1	50.0	57.4	57.8	804-805 bp	1.0	50.1