Hotspot

trnH-GUG-psbA

LSC · 87-555 · 469 bp

Back to Atractylis

Pi 0.00683

MarkerSeek score 51.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 469
variable_sites: 3
pi: 0.00683
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	TCGAAGCTCCATCTACAAATGG	50.0	45.5	57.9	58.2	493-523 bp	1.0	65.8
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	TCGAAGCTCCATCTACAAATGG	50.0	45.5	58.2	58.2	494-524 bp	1.0	65.6
trnH-GUG-psbA_p3	CTGCCTTAATCCACTTGGCT	CGAAGCTCCATCTACAAATGG	50.0	47.6	57.9	56.8	492-522 bp	1.0	65.6
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	CGAAGCTCCATCTACAAATGG	50.0	47.6	58.2	56.8	493-523 bp	1.0	65.3
trnH-GUG-psbA_p5	AATCCACTTGGCTACATCCG	TCGAAGCTCCATCTACAAATGG	50.0	45.5	57.7	58.2	486-516 bp	1.0	64.2