Hotspot

ndhF

SSC · 106004-107593 · 1590 bp

Back to Athyrium

Pi 0.0284

MarkerSeek score 67.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 1590
variable_sites: 137
pi: 0.0284
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGGGATGGGGAAGATGAACA	CCGCGTCAATGTTAGGGTTA	50.0	50.0	58.0	58.0	1911 bp	1.0	86.0
ndhF_p2	AGGGATGGGGAAGATGAACA	TACAAGAAGCTTACGTCGCC	50.0	50.0	58.0	58.0	2210 bp	1.0	85.8
ndhF_p3	GGAGCTGGAAATAAGCACGA	CCGCGTCAATGTTAGGGTTA	50.0	50.0	58.0	58.0	2491-2505 bp	1.0	85.5
ndhF_p4	AGGGATGGGGAAGATGAACA	TTACCGCGTCAATGTTAGGG	50.0	50.0	58.0	58.0	1914 bp	0.230769	49.1
ndhF_p5	GGAGCTGGAAATAAGCACGA	TTACCGCGTCAATGTTAGGG	50.0	50.0	58.0	58.0	2504 bp	0.230769	49.0