Hotspot

trnP-GGG-trnL-UAG

SSC · 110026-110291 · 266 bp

Back to Athyrium

Pi 0.0436

MarkerSeek score 71.80

Primer available yes

Label trnP-GGG-trnL-UAG

Feature Metadata

label_name: trnP-GGG-trnL-UAG
feature_type: igs
region: SSC
length_bp: 266
variable_sites: 34
pi: 0.0436
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-trnL-UAG_p1	GATTCGAACCTGTGACCTCC	CTGGCACTGCTTCCTAAGAG	55.0	55.0	58.0	58.0	378-414 bp	1.0	87.4
trnP-GGG-trnL-UAG_p2	GGATTCGAACCTGTGACCTC	CTGGCACTGCTTCCTAAGAG	55.0	55.0	58.0	58.0	379-415 bp	1.0	87.4
trnP-GGG-trnL-UAG_p3	GATTCGAACCTGTGACCTCC	CTCTGGCACTGCTTCCTAAG	55.0	55.0	58.0	58.0	380-416 bp	1.0	87.3
trnP-GGG-trnL-UAG_p4	GGATTCGAACCTGTGACCTC	CTCTGGCACTGCTTCCTAAG	55.0	55.0	58.0	58.0	381-417 bp	1.0	87.3
trnP-GGG-trnL-UAG_p5	GATTCGAACCTGTGACCTCC	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.0	57.9	367-403 bp	1.0	86.8