Hotspot

trnI-CAU-trnT-UGU

IRb · 82402-83810 · 1409 bp

Back to Athyrium

Pi 0.00726

MarkerSeek score 46.10

Primer available yes

Label trnI-CAU-trnT-UGU

Feature Metadata

label_name: trnI-CAU-trnT-UGU
feature_type: igs
region: IRb
length_bp: 1409
variable_sites: 37
pi: 0.00726
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-trnT-UGU_p1	GCCAATTATGAGTTGGGTGC	TATCTCCCCCGGAATAGACG	50.0	55.0	57.4	57.8	1471-1477 bp	1.0	76.3
trnI-CAU-trnT-UGU_p2	ATGAGTTGGGTGCTTTGACC	TATCTCCCCCGGAATAGACG	50.0	55.0	58.4	57.8	1464-1470 bp	0.923077	74.8
trnI-CAU-trnT-UGU_p3	TCGCCAATTATGAGTTGGGT	TATCTCCCCCGGAATAGACG	45.0	55.0	57.2	57.8	1473-1479 bp	1.0	74.6
trnI-CAU-trnT-UGU_p4	CGCCAATTATGAGTTGGGTG	TATCTCCCCCGGAATAGACG	50.0	55.0	57.2	57.8	1472-1478 bp	1.0	74.2
trnI-CAU-trnT-UGU_p5	TTCGCCAATTATGAGTTGGGT	TATCTCCCCCGGAATAGACG	42.9	55.0	57.9	57.8	1474-1480 bp	1.0	72.3