Hotspot

psaJ-rpl33

LSC · 63423-63655 · 233 bp

Back to Athyrium

Pi 0.0428

MarkerSeek score 71.60

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 233
variable_sites: 29
pi: 0.0428
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACAGCTTCCTTCCTCACTCT	GTAGGTGTGTTACGGCGATT	50.0	50.0	58.0	58.0	1051-1073 bp	1.0	88.2
psaJ-rpl33_p2	TTGGGGGTGATGTAGGAGTT	GTAGGTGTGTTACGGCGATT	50.0	50.0	57.9	58.0	1019-1041 bp	1.0	87.9
psaJ-rpl33_p3	ACAGCTTCCTTCCTCACTCT	AACGTCTACGGAGAGATCGT	50.0	50.0	58.0	58.0	1221-1243 bp	1.0	87.2
psaJ-rpl33_p4	ACAGCTTCCTTCCTCACTCT	ACGTCTACGGAGAGATCGTT	50.0	50.0	58.0	58.0	1220-1242 bp	1.0	87.2
psaJ-rpl33_p5	ACAGCTTCCTTCCTCACTCT	TTCAATCACGTTGCTTTCGC	50.0	45.0	58.0	58.0	1493-1515 bp	0.923077	83.0