Hotspot

rps4-trnL-UAA

LSC · 44705-45380 · 676 bp

Back to Athyrium

Pi 0.0205

MarkerSeek score 65.50

Primer available yes

Label rps4-trnL-UAA

Feature Metadata

label_name: rps4-trnL-UAA
feature_type: igs
region: LSC
length_bp: 676
variable_sites: 46
pi: 0.0205
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-UAA_p1	CGTAAGCCCCGGTAAATTCT	ATTGCCCGATAAATGGTCCC	50.0	50.0	57.7	58.0	825-843 bp	1.0	83.5
rps4-trnL-UAA_p2	CGTAAGCCCCGGTAAATTCT	CTGCCACTTGATACGTCTCC	50.0	55.0	57.7	58.1	784-802 bp	1.0	83.3
rps4-trnL-UAA_p3	CGTAAGCCCCGGTAAATTCT	TCCATACCCAGGCTCAATCT	50.0	50.0	57.7	57.8	767-785 bp	1.0	82.5
rps4-trnL-UAA_p4	CGTAAGCCCCGGTAAATTCT	TCTCCATACCCAGGCTCAAT	50.0	50.0	57.7	57.8	769-787 bp	1.0	82.4
rps4-trnL-UAA_p5	CGTAAGCCCCGGTAAATTCT	CCATACCCAGGCTCAATCTG	50.0	55.0	57.7	57.7	766-784 bp	1.0	81.9