Hotspot

rpl32

Genome · 55052-55852 · 801 bp

Back to Athrotaxis

Pi 0.122

MarkerSeek score 81.90

Primer available yes

Label rpl32

Feature Metadata

label_name: rpl32
feature_type: gene
region: Genome
length_bp: 801
variable_sites: 87
pi: 0.122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32_p1	CACAAACCTCTTTGGGACGA	CGACTCTCGCCGTATGAAAG	50.0	55.0	58.0	58.5	1796-1813 bp	1.0	84.3
rpl32_p2	TTCCAAATTAACGTGGCCCA	CGACTCTCGCCGTATGAAAG	45.0	55.0	58.0	58.5	1909-1926 bp	1.0	84.2
rpl32_p3	TGCATTGATGGATGATGGGG	CGACTCTCGCCGTATGAAAG	50.0	55.0	58.0	58.5	2078-2095 bp	1.0	83.6
rpl32_p4	TCGTTTACGTTTACCCGCAA	CGACTCTCGCCGTATGAAAG	45.0	55.0	58.1	58.5	1821-1838 bp	1.0	83.4
rpl32_p5	ATCATGAGGTCCTTCTCCGT	CGACTCTCGCCGTATGAAAG	50.0	55.0	57.8	58.5	2045-2062 bp	1.0	82.4