Hotspot

ndhJ-chlN

Genome · 130009-130746 · 738 bp

Back to Athrotaxis

Pi 0.0169

MarkerSeek score 72.30

Primer available yes

Label ndhJ-chlN

Feature Metadata

label_name: ndhJ-chlN
feature_type: igs
region: Genome
length_bp: 738
variable_sites: 12
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-chlN_p1	GTTGGATTGGGTGGCCTTTA	ACTTGGGTCCAACCACTTTG	50.0	50.0	58.1	58.2	813-841 bp	1.0	81.7
ndhJ-chlN_p2	TTGGATTGGGTGGCCTTTAC	ACTTGGGTCCAACCACTTTG	50.0	50.0	58.1	58.2	812-840 bp	1.0	81.7
ndhJ-chlN_p3	GTTGGATTGGGTGGCCTTTA	ATTCATGGGTTTGCCAATGC	50.0	45.0	58.1	57.6	889-917 bp	1.0	79.9
ndhJ-chlN_p4	TTGGATTGGGTGGCCTTTAC	ATTCATGGGTTTGCCAATGC	50.0	45.0	58.1	57.6	888-916 bp	1.0	79.9
ndhJ-chlN_p5	TTGGATTGGGTGGCCTTTAC	CCAGATTCATGGGTTTGCCA	50.0	50.0	58.1	58.4	892-920 bp	1.0	79.6