Hotspot

LK118_pgr003-LK118_pgr002

Genome · 121427-121428 · 2 bp

Back to Athrotaxis

Pi 0.5

MarkerSeek score 81.60

Primer available yes

Label LK118_pgr003-LK118_pgr002

Feature Metadata

label_name: LK118_pgr003-LK118_pgr002
feature_type: igs
region: Genome
length_bp: 2
variable_sites: 1
pi: 0.5
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
LK118_pgr003-LK118_pgr002_p1	CCACCTCAAGATGAGTGCTC	TCACTGCACTTCCACTTGAC	55.0	50.0	58.0	58.0	81 bp	1.0	80.4
LK118_pgr003-LK118_pgr002_p2	GAAGCCCACCTCAAGATGAG	TCACTGCACTTCCACTTGAC	55.0	50.0	58.0	58.0	86 bp	1.0	80.3
LK118_pgr003-LK118_pgr002_p3	GCGGATAATTGCTGAATGCC	TCACTGCACTTCCACTTGAC	50.0	50.0	57.9	58.0	115 bp	1.0	79.9
LK118_pgr003-LK118_pgr002_p4	GTACCAGTTATCGTGCCCTC	TCACTGCACTTCCACTTGAC	55.0	50.0	57.8	58.0	162 bp	1.0	78.4
LK118_pgr003-LK118_pgr002_p5	CCACCTCAAGATGAGTGCTC	CACTGCACTTCCACTTGACA	55.0	50.0	58.0	58.3	80 bp	1.0	78.2