Hotspot

LK118_pgr004-trnI-GAU

Genome · 116124-116553 · 430 bp

Back to Athrotaxis

Pi 0.0303

MarkerSeek score 72.80

Primer available yes

Label LK118_pgr004-trnI-GAU

Feature Metadata

label_name: LK118_pgr004-trnI-GAU
feature_type: igs
region: Genome
length_bp: 430
variable_sites: 13
pi: 0.0303
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
LK118_pgr004-trnI-GAU_p1	GACCGGAGTGAAGTCGTAAC	GCAACGACGCAATTATCAGG	55.0	50.0	58.0	57.9	540-545 bp	1.0	81.1
LK118_pgr004-trnI-GAU_p2	GACCGGAGTGAAGTCGTAAC	CGCAACGACGCAATTATCAG	55.0	50.0	58.0	58.3	541-546 bp	1.0	79.6
LK118_pgr004-trnI-GAU_p3	GACCGGAGTGAAGTCGTAAC	AACGACGCAATTATCAGGGG	55.0	50.0	58.0	58.3	538-543 bp	1.0	79.2
LK118_pgr004-trnI-GAU_p4	CAAGGTAGCCGTACTGGAAG	GCAACGACGCAATTATCAGG	55.0	50.0	57.7	57.9	521-526 bp	1.0	79.0
LK118_pgr004-trnI-GAU_p5	AAGTCGTAACAAGGTAGCCG	GCAACGACGCAATTATCAGG	50.0	50.0	57.6	57.9	530-535 bp	1.0	78.5