Hotspot

infA-rps8

Genome · 93229-93572 · 344 bp

Back to Athrotaxis

Pi 0.135

MarkerSeek score 66.40

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: Genome
length_bp: 344
variable_sites: 28
pi: 0.135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.60
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	GACTAGAAACGCTCCACCAG	AGCAGCACCTAATATGCCTC	55.0	50.0	58.0	57.4	1049-1185 bp	1.0	83.0
infA-rps8_p2	GACTAGAAACGCTCCACCAG	GCAGCACCTAATATGCCTCT	55.0	50.0	58.0	57.4	1048-1184 bp	1.0	83.0
infA-rps8_p3	GGAAATCCTGCGATGACTGT	AGCAGCACCTAATATGCCTC	50.0	50.0	58.0	57.4	1030-1166 bp	1.0	82.7
infA-rps8_p4	GGAAATCCTGCGATGACTGT	GCAGCACCTAATATGCCTCT	50.0	50.0	58.0	57.4	1029-1165 bp	1.0	82.7
infA-rps8_p5	ATTTTGCGAACAGAAGCACG	GCAGCACCTAATATGCCTCT	45.0	50.0	58.0	57.4	1301-1437 bp	1.0	82.3