Hotspot

trnM-CAU-atpB

Genome · 56456-56699 · 244 bp

Back to Athrotaxis

Pi 0.0451
MarkerSeek score 68.70
Primer available yes
Label trnM-CAU-atpB

Feature Metadata

label_name
trnM-CAU-atpB
feature_type
igs
region
Genome
length_bp
244
variable_sites
11
pi
0.0451
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpB_p1 GCGAGAGTCGTTGGTTCAAA CCTGCCGTATGCTCAAAAGA 50.0 50.0 58.8 58.3 330-331 bp 1.0 74.3
trnM-CAU-atpB_p2 GCTTTCATACGGCGAGAGTC CCTGCCGTATGCTCAAAAGA 55.0 50.0 58.8 58.3 341-342 bp 1.0 74.1
trnM-CAU-atpB_p3 AGAGTATCGCTTTCATACGGC CCTGCCGTATGCTCAAAAGA 47.6 50.0 58.0 58.3 349-350 bp 1.0 72.3
trnM-CAU-atpB_p4 GCGAGAGTCGTTGGTTCAA CCTGCCGTATGCTCAAAAGA 52.6 50.0 58.1 58.3 330-331 bp 1.0 71.7
trnM-CAU-atpB_p5 GCGAGAGTCGTTGGTTCAAA ATCTGAGTAATACGGCCTGC 50.0 50.0 58.8 57.2 345-346 bp 1.0 69.7