Hotspot

rpl14

LSC · 84304-84672 · 369 bp

Back to Atalantia

Pi 0.0105

MarkerSeek score 38.90

Primer available yes

Label rpl14

Feature Metadata

label_name: rpl14
feature_type: gene
region: LSC
length_bp: 369
variable_sites: 11
pi: 0.0105
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14_p1	TCAGCAATAGTGTCCTTGCC	TGAGTCCTTTCATTCGCGAG	50.0	50.0	57.9	58.0	1172-1212 bp	1.0	85.6
rpl14_p2	TCACTATATTGCGGTCCCCT	TGAGTCCTTTCATTCGCGAG	50.0	50.0	57.9	58.0	1020-1043 bp	1.0	85.3
rpl14_p3	TCCTATTCGAACCGTTCCCT	TGAGTCCTTTCATTCGCGAG	50.0	50.0	58.1	58.0	1230-1270 bp	0.875	79.6
rpl14_p4	AAAAGAACCCGGAGCATACC	TGAGTCCTTTCATTCGCGAG	50.0	50.0	57.9	58.0	1679-1716 bp	0.875	78.4
rpl14_p5	TCGTCGATGTCGTAGGGTTA	TGAGTCCTTTCATTCGCGAG	50.0	50.0	58.0	58.0	1366-1390 bp	0.375	56.0