Hotspot

ndhF-rpl32

SSC · 116620-117651 · 1032 bp

Back to Atalantia

Pi 0.0257
MarkerSeek score 72.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1032
variable_sites
72
pi
0.0257
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAACTAAAGGTATGATCCAGGA TCCAAAGATTTTTACGAATACG 43.5 31.8 57.1 52.9 1116-1177 bp 1.0 48.5
ndhF-rpl32_p2 GGAACTAAAGGTATGATCCAGGA TTCCAAAGATTTTTACGAATACG 43.5 30.4 57.1 53.8 1117-1178 bp 1.0 48.5
ndhF-rpl32_p3 GGAACTAAAGGTATGATCCAGGAA TCCAAAGATTTTTACGAATACG 41.7 31.8 57.8 52.9 1116-1177 bp 1.0 48.5
ndhF-rpl32_p4 GGAACTAAAGGTATGATCCAGGA TTTCCAAAGATTTTTACGAATACG 43.5 29.2 57.1 54.5 1118-1179 bp 1.0 48.5
ndhF-rpl32_p5 GGAACTAAAGGTATGATCCAGG TCCAAAGATTTTTACGAATACG 45.5 31.8 55.7 52.9 1116-1177 bp 1.0 48.5