Hotspot

psbT-pbf1

LSC · 77935-77999 · 65 bp

Back to Atalantia

Pi 0.0621

MarkerSeek score 66.20

Primer available yes

Label psbT-pbf1

Feature Metadata

label_name: psbT-pbf1
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 9
pi: 0.0621
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-pbf1_p1	TCGAGAACCGCCTAAAGTTC	GATCCATTCGAGGAACACGG	50.0	55.0	57.6	58.4	123-134 bp	1.0	83.2
psbT-pbf1_p2	TCGAGAACCGCCTAAAGTTC	GGGTACGCCTTATATACCGC	50.0	55.0	57.6	57.7	171-182 bp	1.0	82.9
psbT-pbf1_p3	GAGAACCGCCTAAAGTTCCA	GATCCATTCGAGGAACACGG	50.0	55.0	57.5	58.4	121-132 bp	1.0	82.4
psbT-pbf1_p4	GAGAACCGCCTAAAGTTCCA	GGGTACGCCTTATATACCGC	50.0	55.0	57.5	57.7	169-180 bp	1.0	82.1
psbT-pbf1_p5	TCGAGAACCGCCTAAAGTTC	GGCAACCCTCTCAACAACTA	50.0	50.0	57.6	57.4	146-157 bp	1.0	81.3