Hotspot

psbE-petL

LSC · 68042-69431 · 1390 bp

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Pi 0.0129

MarkerSeek score 70.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1390
variable_sites: 49
pi: 0.0129
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCCGCTAGTAGAAAACCAA	50.0	45.0	57.9	56.9	1443-1543 bp	1.0	77.1
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCA	50.0	45.0	57.9	56.9	1444-1544 bp	1.0	77.0
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCA	50.0	45.0	57.8	56.9	1367-1467 bp	1.0	76.4
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AGCCGCTAGTAGAAAACCAA	50.0	45.0	57.8	56.9	1366-1466 bp	1.0	76.4
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AGCCGCTAGTAGAAAACCAA	45.0	45.0	58.2	56.9	1452-1552 bp	1.0	76.1