Hotspot

atpF-atpH

LSC · 13464-13906 · 443 bp

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Pi 0.0749

MarkerSeek score 72.40

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 443
variable_sites: 83
pi: 0.0749
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TGGGTCCAGCCTATTCTTGA	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.0	58.0	481-884 bp	1.0	91.4
atpF-atpH_p2	TGGGTCCAGCCTATTCTTGA	AAATCCTGTGTTGTGCCTGT	50.0	45.0	58.0	57.9	716-1109 bp	1.0	90.7
atpF-atpH_p3	TGGGTCCAGCCTATTCTTGA	TGGGCTTGCTTCTATTGGAC	50.0	50.0	58.0	57.9	443-846 bp	1.0	90.7
atpF-atpH_p4	GTGCTGTGTCTGGACTTCAA	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.0	58.0	692-1101 bp	1.0	90.5
atpF-atpH_p5	GTGCTGTGTCTGGACTTCAA	AAATCCTGTGTTGTGCCTGT	50.0	45.0	58.0	57.9	927-1326 bp	1.0	89.5