Hotspot

ycf1

SSC · 124668-130160 · 5493 bp

Back to Astrocaryum

Pi 0.00333

MarkerSeek score 40.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5493
variable_sites: 60
pi: 0.00333
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AATGATTCGGGTTTCAGTGT	GGCAGTGGATTCATTGACGA	40.0	50.0	55.3	58.3	183 bp	1.0	62.5
ycf1_p2	AAATGATTCGGGTTTCAGTGT	GGCAGTGGATTCATTGACGA	38.1	50.0	56.1	58.3	184 bp	1.0	60.9
ycf1_p3	AATGATTCGGGTTTCAGTGT	TGGCAGTGGATTCATTGACG	40.0	50.0	55.3	58.5	184 bp	1.0	60.2
ycf1_p4	TGATTCGGGTTTCAGTGTATTGA	GGCAGTGGATTCATTGACGA	39.1	50.0	58.1	58.3	181 bp	1.0	59.7
ycf1_p5	AAAATGATTCGGGTTTCAGTGT	GGCAGTGGATTCATTGACGA	36.4	50.0	56.8	58.3	185 bp	1.0	58.8