Hotspot

rpl16

LSC · 81536-83064 · 1529 bp

Back to Astrocaryum

Pi 0.00376

MarkerSeek score 59.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1529
variable_sites: 20
pi: 0.00376
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCAATGCAAAGGATGAAGGA	AGGTTGAATAAAAATTCGATTGACCA	40.0	30.8	54.9	58.1	1674-1732 bp	1.0	43.3
rpl16_p2	TCAATGCAAAGGATGAAGGA	GGTTGAATAAAAATTCGATTGACCA	40.0	32.0	54.9	56.9	1673-1731 bp	1.0	43.3
rpl16_p3	TCAATGCAAAGGATGAAGGAA	AGGTTGAATAAAAATTCGATTGACCA	38.1	30.8	55.7	58.1	1674-1732 bp	1.0	43.3
rpl16_p4	TTCAATGCAAAGGATGAAGGA	AGGTTGAATAAAAATTCGATTGACCA	38.1	30.8	55.7	58.1	1675-1733 bp	1.0	43.3
rpl16_p5	TCAATGCAAAGGATGAAGGAA	GGTTGAATAAAAATTCGATTGACCA	38.1	32.0	55.7	56.9	1673-1731 bp	1.0	43.3