Hotspot

psbA

LSC · 222-1280 · 1059 bp

Back to Astrocaryum

Pi 0.00267

MarkerSeek score 35.70

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1059
variable_sites: 24
pi: 0.00267
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TCAACGATTCATATACACTAAGACA	ATTGAATAAACCAAGATCTTACC	32.0	30.4	55.9	52.5	1113-1116 bp	0.941176	39.4
psbA_p2	TCAACGATTCATATACACTAAGACA	AATTGAATAAACCAAGATCTTACC	32.0	29.2	55.9	53.3	1114-1117 bp	0.941176	39.4
psbA_p3	CAACGATTCATATACACTAAGACA	ATTGAATAAACCAAGATCTTACC	33.3	30.4	54.6	52.5	1112-1115 bp	0.941176	39.4
psbA_p4	TCAACGATTCATATACACTAAGACA	TGAAAATTGAATAAACCAAGATCT	32.0	25.0	55.9	53.2	1118-1121 bp	0.941176	39.4
psbA_p5	TCAACGATTCATATACACTAAGACAA	ATTGAATAAACCAAGATCTTACC	30.8	30.4	56.6	52.5	1113-1116 bp	0.941176	39.4