Hotspot

rpl32-trnL-UAG

SSC · 114121-114717 · 597 bp

Back to Astrocaryum

Pi 0.00765
MarkerSeek score 60.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
597
variable_sites
19
pi
0.00765
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGTTTCTACCGGACATTCAA AGCGTGTCTACCGATTTCAC 45.0 50.0 55.4 58.0 666-893 bp 1.0 64.7
rpl32-trnL-UAG_p2 GGTTTCTACCGGACATTCAA CAGCGTGTCTACCGATTTCA 45.0 50.0 55.4 58.0 667-894 bp 1.0 64.7
rpl32-trnL-UAG_p3 GGTTTCTACCGGACATTCAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 55.4 57.9 676-903 bp 1.0 63.8
rpl32-trnL-UAG_p4 GGTTTCTACCGGACATTCAA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 55.4 57.8 675-902 bp 1.0 63.2
rpl32-trnL-UAG_p5 GGTTTCTACCGGACATTCAA CTAAGAGCAGCGTGTCTACC 45.0 55.0 55.4 57.8 674-901 bp 1.0 63.2