Hotspot

rbcL-accD

LSC · 57108-57905 · 798 bp

Back to Astrocaryum

Pi 0.00499

MarkerSeek score 45.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 798
variable_sites: 12
pi: 0.00499
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCAAATGGAGCCCTGAACTA	GGGTTCTTCACTTCCACTGG	50.0	55.0	57.9	58.1	1007-1016 bp	1.0	79.3
rbcL-accD_p2	TAGCAAATGGAGCCCTGAAC	GGGTTCTTCACTTCCACTGG	50.0	55.0	57.9	58.1	1009-1018 bp	1.0	79.3
rbcL-accD_p3	TGAAGCTAGCAAATGGAGCC	GGGTTCTTCACTTCCACTGG	50.0	55.0	58.2	58.1	1015-1024 bp	1.0	78.3
rbcL-accD_p4	CCGTGAAGCTAGCAAATGGA	GGGTTCTTCACTTCCACTGG	50.0	55.0	58.3	58.1	1018-1027 bp	1.0	78.2
rbcL-accD_p5	TCCGTGAAGCTAGCAAATGG	GGGTTCTTCACTTCCACTGG	50.0	55.0	58.3	58.1	1019-1028 bp	1.0	78.2