Hotspot

trnT-GGU-psbD

LSC · 31897-32892 · 996 bp

Back to Astrocaryum

Pi 0.00195

MarkerSeek score 50.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 996
variable_sites: 8
pi: 0.00195
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AACGGGCCCTTTTAACTCAG	GCCAGACCAGCCTACAAAAA	50.0	50.0	58.1	58.4	1162-1184 bp	1.0	78.0
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	GCCAGACCAGCCTACAAAAA	50.0	50.0	57.9	58.4	1157-1179 bp	1.0	78.0
trnT-GGU-psbD_p3	AACGGGCCCTTTTAACTCAG	AAAGAGCAATAGGCCAGACC	50.0	50.0	58.1	57.6	1174-1196 bp	1.0	77.7
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	AAAGAGCAATAGGCCAGACC	50.0	50.0	57.9	57.6	1169-1191 bp	1.0	77.6
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	GCCAGACCAGCCTACAAAAA	50.0	50.0	58.3	58.4	1120-1142 bp	1.0	76.5